Sapidyne Instruments Inc.

Investigations of Influence of Antibody Binding Kinetics on Tumor Distribution and Anti-Tumor Efficacy

"An important aspect of this area of research is the selection of an appropriate binding assay to accurately characterize the binding affinity and kinetics of the antibodies. Techniques commonly employed in binding assays, such as ELISA, flow cytometry, KinExA, and SPR, have their unique attributes and limitations. Among these, KinExA stands out for its applicability in quantifying high-affinity interactions. It works by measuring the concentration of free (i.e., unbound) ligand in a binding reaction that has reached equilibrium prior to the instrument analysis, effectively bypassing potential artifacts resulting from non-equilibrium conditions, ligand depletion, and mass transport effects. Such artifacts may lead to significant errors in other assays like ELISA or SPR (33, 34). Additionally, KinExA facilitates the direct measurement of binding kinetics on antigen-expressing cells, reflecting real and actual binding conditions (35, 36). Consequently, KinExA was chosen to characterize the binding affinity and kinetics of all the IgGs investigated in this study."

Investigations of Influence of Antibody Binding Kinetics on Tumor Distribution and Anti-Tumor Efficacy - The AAPS Journal The pharmacokinetics of antibodies with varied binding kinetics were simulated to assess the role of affinity and binding microconstants (kon, koff) on tumor exposure and intra-tumoral distribution. Anti-HER2 constructs (trastuzumab, pertuzumab, VK3VH6, and conjugates with DM1 and gelonin) were prod...

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