Cutting Edge Biotechnology

New England Biolabs Inc. has published this article which describes, in detail, the methodology of the CRISPR Cas9 system. Hope you can all take something useful away from it!

https://www.neb.com/tools-and-resources/feature-articles/crispr-cas9-and-targeted-genome-editing-a-new-era-in-molecular-biology

CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology | NEB The development of efficient and reliable ways to make precise, targeted changes to the genome of living cells is a long-standing goal for biomedical researchers. Recently, a new tool based on a bacterial CRISPR-associated protein-9 nuclease (Cas9) from Streptococcus pyogenes has generated considera...