MLS

HOW TO PREPARE CULTURE MEDIA?

-Weigh the amount of ingredients powder on weighing machine.

-Dissolve the ingredients in distilled water.

-Adjust PH of the medium if needed.

-Add agar and boiled it to dissolve.

-Pour the media into flask.

-Autoclave the media when ingredients fully dissolve.

-Sterilization is done in autoclave to prevent from contamination, at 121ºC for 15 min at 15lbs.

-After the autoclave place the media flask in laminar air flow.

-Sterilize the laminar air flow with 70% alcohol.

-A bit cools down the media and pours into sterile Petri-plates for solidification.

-Then sample is ready to spread(spreader) / streak

-(Inoculation loop) on the medium for identification or isolation of microbes.

-Sealed the Petri plates with paraffin, label them.

-Keep them inverted in incubator at 37ºC for 24hrs.

-Observe the result next day colonies formation is visible on the media.